Fluorescence-guided surgery is recognized in Latin America but remains inconsistently applied across surgical specialties, primarily due to economic and logistical barriers.

• Adoption is mostly in high-resource, specialized centers, with practical use seen in biliary, breast, urologic, and gastrointestinal surgeries.
• Barriers include limited operating room access, high costs of equipment and fluorophores, and unclear reimbursement policies, hindering broader implementation.

Surgeons express positive perceptions of safety and benefits, indicating strong interest in regional collaboration for data sharing and registry participation.